Gibson & Lanni

The Gibson & Lanni model assumes that, disregarding defocus, all observed aberrations are generated by factors external to the objective (i.e. originating in the sample, coverslip and immersion medium combination). These aberrations can be characterized by the optical path difference between a ray in a perfect system (see BornWolf) and a ray under experimental conditions.

TransferFunctions.GibsonLanni — Type

GibsonLanni{T<:Real} <: PSFModel{2}

Gibson & Lanni model point spread function

Fields

λ::Length{T}: emission wavelength
NA::T: numerical aperture of the objective
n_i::T: refractive index of the immersion medium
n_s::T: refractive index of the sample
n_g::T: refractive index of the coverslip
t_i::Length{T}: working distance of the objective
t_g::Length{T}: coverslip thickness

source

TransferFunctions.GibsonLanni — Method

GibsonLanni(λ::Length, NA, n_i=1.5, n_s=4//3, n_g=1.5, t_i, t_g)

GibsonLanni model point spread function with emission wavelength λ, numerical aperture NA, immersion medium refractive index n_i, sample refractive index n_s, coverslip refractive index n_g, working distance t_i and coverslip thickness t_g.

source